tdTomato mRNA (unmodified)
tdTomato mRNA is a synthetic mRNA that encodes tdTomato, a bright red fluorescent protein. It is widely used in molecular and cell biology research for real-time visualization of cellular processes. tdTomato mRNA emits light at 581 nm and is ideal for tracking gene expression, protein localization, and live cell imaging.
Croyez’s tdTomato mRNAs was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.
Croyez’s tdTomato mRNAs was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.
mRNA Length:
2035 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
2035 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
tdTomato mRNA
Figure 1. 293T cells (0.8 × 10⁵ cells per well, 24-well plate) were transfected with 1 µg of tdTomato mRNA using the EndoSafe mRNA Transfection Kit (C15053-K01).
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Figure 2. tdTomato mRNA was analyzed on a 2% E-Gel,
Marker : Croyez High Range RNA ladder (cat no: CR00005)
Marker : Croyez High Range RNA ladder (cat no: CR00005)
Figure 3. tdTomato mRNA was analyzed on a 1% TAE agarose gel at 100 V for 30 minutes
Figure 4. Capillary Electrophoresis
tdTomato mRNA was analyzed by capillary electrophoresis
